I forgot to take the LB media from the autoclave last night. When I arrived today it was dark amber and each flask had lost ~100mL to evaporation. Is my media burnt and has to be discarded, or can I reconstitute it adding autoclaved water?

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Comment by Madeline (Brigit) on October 15, 2009 at 3:43pm
Thank you! To think that it was like 5 liters...

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