Madeline (Brigit)'s Blog (8)

I think I burned my LB media

I forgot to take the LB media from the autoclave last night. When I arrived today it was dark amber and each flask had lost ~100mL to evaporation. Is my media burnt and has to be discarded, or can I reconstitute it adding autoclaved water?

Added by Madeline (Brigit) on October 15, 2009 at 11:52am — 1 Comment

Get your proposal-writing fingers ready!

This just came out in my inbox and I though it might be useful to someone else needing funding.



Sponsor – Bill and Melinda Gates Foundation - Grand Challenges Explorations (GCE)



Description – GCE supports hundreds of early-stage research projects - including many ideas that have never before been tested -- and scientists from a wide range of disciplines and regions. The Explorations initiative funds innovative ideas that could lead to new vaccines, diagnostics, drugs,…
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Added by Madeline (Brigit) on October 12, 2009 at 10:00am — No Comments

When mom knows too little about sex...

This kind of stuff results:
epic fail pictures
see more Epic Fails

Added by Madeline (Brigit) on October 7, 2009 at 1:30pm — 4 Comments

Happy Blasphemy Day!

To celebrate, I'm rocking at the lab with some awesome blasphemous music.

First, the awesomeness that is El Ultimo ke… Continue

Added by Madeline (Brigit) on October 1, 2009 at 12:30pm — 1 Comment

Know of any medical charities that work in Congo?

Hi all,



The people over at the Lola ya Bonobo Sanctuary in Congo have been approached by the family of a little 7 year old named Amazone. She has a Huge head tumor. the tumor covers a large part of her right side of the face. I doubt she has any eye function on that side. Please ask around to see if anyone knows some way to help, and give all information to the people over there. Or here and I'll post… Continue

Added by Madeline (Brigit) on September 2, 2008 at 9:19pm — 2 Comments

Cheesus found!!

You lucky folks from Missouri, the power of Cheesus is in your state! Just look at him

This has finally proven that some folks began to put him in the shroud before he was taken off the cross. They didn't have all day ya' know? His head is very small, which is not at all surprising, but he apparently rocked out a ponytail years before it was… Continue

Added by Madeline (Brigit) on July 30, 2008 at 11:23am — 5 Comments

The lab pixies strike again

My protein, a dark, evil mistress from hell, refused to fold on command like her nicely behaving homologous sister. A year into assaying folding and buffer conditions with only slight improvement, I went the mutagenesis route.



After doing all sort of skippy victory dances when I finally got my mutant, I transformed some nice bacteria, grew them and burst them. So I'm, at last, folding the protein again. I expect no problems, I tried folding some of my incomplete mutant and it… Continue

Added by Madeline (Brigit) on July 28, 2008 at 9:02am — No Comments

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